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In silico titration of two human genomes. Blue represents reads from NA12878 (designated normal). Red represents reads from NS12911 (designated tumor). Going from (a) to (b) represents a somatic mutation of G >A, where G in the normal is a homozygous reference and A in the tumor is a heterozygous variant. c A normal contaminated with tumor tissues. d A tumor sample contaminated with normal tissues
