Table 1.

Example use cases for process control mixtures

User	Sample design	Time to use	Benefit
Technology and Experimental Protocol Developers	Mix 2–3 components of biological interest appropriate for use to evaluate the experimental protocol/technology	When validating experimental protocols and/or technology platforms	Demonstrate the ability of a protocol and/or technology platform to consistently measure transcripts of interest
Core Labs	Repeated measures of highly-available samples	After changes in technician, prototol, reagents, or technology platform, etc.	Show internal consistency of output and linearity of measurements
			Identify biases
			Understand dispersion
Informaticians	Relevant samples, public data (eg: SEQC, this study)	When comparing informatics analysis tools or developing new tools	Use non-simulated benchmark datasets to determine how accurate results are.