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. 2015 Sep 17;10(9):e0138190. doi: 10.1371/journal.pone.0138190

Fig 6. Cell surface expression of BST2 variants.

Fig 6

293T and CrFK cells were co-transfected with 500 ng of VR1012, fBST2, fBST2 N79A, fBST2 N119A, fBST2 N79/119A, fBST2-IHA, fBST2-NHA or fBST2* expression plasmid, along with 500 ng of pEGFP-N3 as a transfection marker. After 48 h, cells were harvested and stained with the anti-fBST2 pAb and Alexa 633 goat anti-rabbit IgG, followed by flow cytometric analysis. Cells only transfected with pEGFP-N3 were used as a negative control. The samples were gated on EGFP+ cells, and the surface BST2 levels are shown in the column diagram with mean fluorescent intensity values (A and B). This experiment was repeated three times, and the most representative data are shown.