Fig. 2.

GBM models sensitive to V-4084 share common genetic profiles. a Unsupervised hierarchical clustering was performed on tumor samples from Fig. 1d; three tumors from each group were used for analysis. Sensitive tumors (U87M2, U118, and SF295SQ1) clustered together, away from the insensitive ones (U251M2 and DBM2). Within the most sensitive tumors (U87M2 and U118), there was a clear separation between V4084-treated and vehicle-treated samples. b Principal component analysis (PCA) corroborated the results shown in panel A. All sensitive tumors were closer to each other and farther from the insensitive tumors. Note that SF295 showed partial sensitivity to V4084 and lies between the two phenotypes. c Tumors sensitive and insensitive to V-4084 were analyzed by microarray. We identified 301 differentially expressed genes (Student’s t test, p ≤ 0.005). While SF295 was not included in the analysis due to its partial sensitivity, it is in the heatmap between the yellow lines. Although clustering with the sensitive cell lines, SF295 tumors share similarities with the insensitive tumors