FIG. 4.

Effects of GANT61 on Gli1-LacZ (A, B, I, and J), Gli2-LacZ (C, D, K, and L), and Scc (E, F, M, and N) expression in gonad explant culture are shown. E11.5 (Treatment 1 as shown in Fig. 2) or E12.5 (Treatment 2) testes were cultured for 72 h in the presence of either DMSO or GANT61. After culture, samples were processed for LacZ staining, whole-mount in situ hybridization for Scc, or immunohistochemistry for AMH (red) and 3βHSD (green in panels G and H). All culture experiments were repeated three times, and in each experiment, at least three gonads were exposed to the same treatment. A–F and I–N) Bar = 500 μm; (G and H) bar = 200 μm.