Fig. 7.

Quantification of cilia-driven tracheal fluid flow in adolescent mouse using DPIV. (a) 3D intensity-based rendering of trachea, with sagittal plane orientation shown (b) Segmented region of trachea within blue box of (a) showing spatial location where fluid flow was quantified. (c, Visualization 7 ^{(2.3MB, MP4)} ) Raw acquisition of 5 μm polystyrene microspheres being driven by ciliary fluid flow, and subsequent 2D2C flow field quantification with DPIV. The acqusition is oblique to the sagittal plane by 15° (d, Visualization 8 ^{(1.1MB, MP4)} ) 3D3C flow field rendering from top view (consistent with view in (a)), oblique superior view, and side view. Large arrows denoting the location and direction of primary tail-head flow as well as recirculatory head-tail flow. Axes labels: L-left, R-right, Cr-cranial, Ca-caudal, D-dorsal, V-ventral