Table 1.
ApoC-I, apoC-II and apoC-III proteoforms, with their relative percent abundance (RPA) and frequency in a cohort of 82 human EDTA-plasma samples
| Proteoforms | Theoretical m/z value |
Observed m/z value |
RPA (%) Mean (range) |
Frequency (%) |
|---|---|---|---|---|
| des-TP apoC-I | 6432.344 | 6432.104 | 21.3 (13.6 – 27.0) | 100 |
| apoC-I native | 6630.565 | 6630.572 | 78.7 (72.9 – 86.4) | 100 |
| des-TQQPQQ apoC-II | 8204.162 | 8205.290 | 8.51 (4.09 – 13.7) | 100 |
| apoC-II native | 8914.906 | 8915.760 | 91.5 (86.3 – 95.9) | 100 |
| apoC-III native | 8764.652 | 8764.587 | 6.85 (1.06 – 19.0) | 100 |
| apoC-III-(Gal)1(GalNAc)1 | 9135.800 | 9137.925 | 29.9 (25.4 – 36.8) | 100 |
| des-A apoC-III-(Gal)1(GalNAc)1(NeuAc)1 | 9350.171 | 9351.781 | 1.17 (0.336 – 3.65) | 93.9 |
| apoC-III-(Gal)1(GalNAc)1(NeuAc)1 | 9422.249 | 9422.562 | 40.6 (29.1 – 48.3) | 100 |
| des-A apoC-III-(Gal)1(GalNAc)1(NeuAc)2 | 9641.429 | 9642.267 | 5.96 (4.32 – 9.26) | 100 |
| apoC-III-(Gal)1(GalNAc)1(NeuAc)2 | 9712.507 | 9713.492 | 12.1 (6.63 – 19.4) | 100 |
| apoC-III-(Gal)2(GalNAc)2(Fuc)3 | 9933.768 | 9933.941 | 2.61 (0.477 – 6.83) | 100 |
| apoC-III-(Gal)3(GalNAc)3(Fuc)2 | 10152.961 | 10153.559 | 0.949 (0.241 – 1.42) | 12.2 |
| apoC-III-(Gal)4(GalNAc)2(Fuc)3 | 10258.056 | 10259.700 | 1.74 (0.139 – 3.78) | 20.7 |
| apoC-III-(Gal)2(GalNAc)4(Fuc)3 | 10340.158 | 10341.098 | 1.40 (0.837 – 2.00) | 17.1 |
| apoC-III-(Gal)3(GalNAc)3(Fuc)4 | 10445.869 | 10446.206 | 0.507 (0.209 – 0.771) | 15.9 |
| apoC-III-(Gal)5(GalNAc)3(Fuc)4 | 10769.541 | 10770.404 | 0.924 (0.593 – 1.53) | 12.2 |
Gal – Galactose; GalNAc - N-acetylgalactosamine; NeuAc - N-acetyl neuraminic acid (sialic acid); Fuc – Fucose