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. 2014 Aug 2;26(12):685–695. doi: 10.1093/intimm/dxu078

Fig. 5.

Fig. 5.

T effector memory cells directed at distinct sets of epitopes. C56BL/6 mice (n = 8) or ERAP1−/− mice (n = 8) were immunized I.M. in the tibialis anterior with viral particles of Ad5-C. difficile-TA (1×1010). At day 21, mice were sacrificed and splenocytes were harvested and stimulated for 6h at 37°C with the labeled peptides. (A) Representative sample demonstrating our gating strategy. (B) Representative example of IFN-γ-, TNF-α-producing splenic CD8+ CD62L− CD127+ T cells stimulated with the WT IDTAP and percentage of IFN-γ-+, TNF-α+ TEM cells. (C) Representative example of IFN-γ-, TNF-α-producing splenic CD8+ CD62L− CD127+ T cells stimulated with the ERAP1−/− IDTAP and percentage of IFN-γ+, TNF-α+ TEM cells. The bars represent means ± SEM. Statistical analysis was completed using one-way ANOVA with a Student–Newman–Keuls post hoc test. A P value < 0.05 was statistically significant. ***P < 0.001, statistically significant from the other three groups of animals.