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. 2015 Oct;21(10):1770–1780. doi: 10.1261/rna.052555.115

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© 2015 Schwer et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 7. — Genetic and functional interaction of Ssu72 with the Pol2 CTD. (A) Exponentially growing cultures of S. pombe strains with the indicated chromosomal rpb1-CTD and ssu72 alleles were adjusted to A₆₀₀ of 0.1, and aliquots of serial fivefold dilutions were spotted to YES agar and incubated at the indicated temperatures. (B) Fission yeast cells with genotypes as specified were assayed for Pho1 activity after incubation for 3 h in PMG medium containing 15.5 mM phosphate (+ phosphate) or lacking exogenous phosphate (− phosphate).