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. 2015 Oct;21(10):1790–1806. doi: 10.1261/rna.052712.115

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© 2015 Apostolidi et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 6. — Chemical probing of the T275:tRNA^Gly complex using a (A) proteinogenic (P1) and a (B) nonproteinogenic (NP2) tRNA^Gly; (S) indicates strong and (W) weak base modifications by DMS or KE; (C) indicates the unmodified control reaction. Primers TbGL_1 and TbGl_6 (Supplemental Table 1) were used for stem I and antiterminator region analysis, respectively. (C) Predicted antiterminator structure and base modification protection by tRNA^Gly binding. Filled black stars correspond to specific protection by P1 tRNA^Gly(GCC) and open stars to protection by NP2 tRNA^Gly(UCC). Highly conserved (100%) and moderately conserved (66%) nucleotides are indicated with gray filled or open circles, respectively.