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. 2015 Sep 17;10(9):e0138473. doi: 10.1371/journal.pone.0138473

Fig 2. Start codon selectivities at haloarchaeal transcripts in the presence and absence of short 5’-UTR.

Fig 2

(A) The 203-bp DNA sequence preceding the bgaH ORF in plasmid pTMJ was identical to the upstream sequence of hsp70 ORF in Natrinema sp. J7. The bases at the 5’-end of transcripts were shown schematically. Their start codons were underlined and the mutations were in grey. The β-galactosidase specific activities (BgaH activities), the bgaH transcript levels and the translational efficiencies of Hfx. volcanii transformants were tabulated. (B) The translational efficiencies of (A) were shown schematically after normalization to that of Hfx. volcanii DS70/pTMJ. (C) The expression of the BgaH protein. Western blot analysis of the BgaH protein in total proteins was performed using anti-BgaH antibody. The Hfx. volcanii transformants were cultivated for 5 days at 45°C and then sprayed with X-Gal. The constructs present in each transformant were indicated under the colonies.