Table 1.
Coronary venous rates of release of glycocalyx constituents (ng min–1 g–1 heart tissue) induced by 10 min intracoronary infusion of various enzymes in guinea pig isolated perfused hearts. Values are means ± SD; for perfusion and analytical details, see [135]
| Enzyme | n | Syndecan-1 | Heparan sulphate | Hyaluronic acid |
|---|---|---|---|---|
| Control† | 9 | 11.7 ± 7.1 | 654 ± 254 (n = 8) | 7.4 ± 6.7 |
| Thrombin‡ | 8 | 5.2 ± 4.3 | 1022 ± 627 (n = 5) | 27.9 ± 32.0* |
| Tryptase§ | 11 | 4.2 ± 3.2 | 804 ± 327 (n = 5) | 19.0 ± 13.9 |
| Cathepsin B¶ | 12 | 4.3 ± 2.6 | 1556 ± 666 (n = 5)* | 30.4 ± 19.3* |
| Elastase** | 6 | 2.6 ± 2.8 | 1413 ± 70 (n = 3)* | 55.6 ± 39.3* |
| Proteinase 3†† | 3 | 2.8 ± 2.2 | (n = 0) | 27.1 ± 17.1 * |
| Plasmin‡‡ | 4 | 2.5 ± 1.3 | (n = 0) | 40.1 ± 16.5* |
| tPA§§ | 3 | 1.7 ± 0.9 | (n = 0) | 15.0 ± 10.3 |
| Hyaluronidase¶¶ | 4 | 2.4 ± 1.5 | (n = 0) | 1197.0 ± 524.7* |
†Control = time matched perfusion without exogenous enzyme, ‡ Thrombin from human plasma (Sigma,USA), 3.3-50U/heart. § Tryptase purified from human lung (Sigma, USA), 2–10 mU, ¶ Cathepsin B from bovine spleen (Sigma, USA), 1.1–5.4U, ** Elastase from human leukocytes (Sigma, USA), 0.33–0.67U, †† Proteinase 3 from human neutrophilic granulocytes (Biomol international, Hamburg,Germany), 0.47U, ‡‡ Plasmin from human plasma (Sigma, USA), 1.5U, §§ Tissue-type plasminogen activator, recombinant human, expressed in CHO cells (Sigma, USA), 1667U, ¶¶ Hyaluronidase from bovine testes (Sigma, USA), 5400U; n = number of hearts; * = P < 0.05 vs. control, ANOVA and unpaired t-test with Bonferroni correction.