Fig 2. GacA positively controlled IAA biosynthesis.

HPLC analysis was performed to detect IAA production with 50 μg/ ml synthetic IAA (A) as a standard between the wild type G5 (B) and the gacA mutant G5-6 (C). 20 μl of each sample were loaded and eluted isocratically at a 1 ml/ min flow rate.