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. 2015 Sep 17;10(9):e0138037. doi: 10.1371/journal.pone.0138037

Fig 7. Effects of chronic running exercise on nitrotyrosine protein expression in the renal cortex and outer medulla of Zucker diabetic fatty rats.

Fig 7

Protein expression of the nitrotyrosine in the renal cortex (A) and outer medulla (B) of sedentary lean Zucker diabetic fatty (ZDF) rats (Sed-ZL), sedentary ZDF rats (Sed-ZDF) and aerobic treadmill exercised ZDF rats (Ex-ZDF) groups were examined by western blot analysis. Top panels depict representative immunoblots from the different groups. The intensities of the nitrotyrosine bands (70 kDa) for the protein were normalized against that of β-actin (40 kDa). The intensity of the band in the Sed-ZL group was assigned a value of 1. Values are means ± SEM (n = 5/group). ** p<0.01 compared with the Sed-ZL group; † p<0.05, †† p<0.01 compared with the Sed-ZDF group.