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. 2015 Sep 17;10(9):e0136556. doi: 10.1371/journal.pone.0136556

Fig 1. 18O-labeling analysis of phosphometabolites using HPLC and 18O-assisted GC/MS.

Fig 1

A, Sample preparation and fractionation for GC/MS analysis. B, Analysis of Pi, G3P, and G6P using GC/MS. C, Enzymatic reactions for γ- and β-ATP and CrP to analyze their 18O-metabolic–labeling ratio with GC/MS as G3P. AK indicates adenylate kinase; CrP, creatine phosphate; G3P, glycerol-3-phosphate; G6P, glucose-6-phosphate; Pi, inorganic phosphate.