Table 2. Estimates of DNA sequence polymorphism and test of neutrality at the nonrepeat terminals and Th2R/Th3R epitope region of P. knowlesi CSP gene.

N = total number of sequences; S = segregating sites; H = number of haplotypes; Hd = haplotype diversity; π = observed average pairwise nucleotide diversity; dN–dS = rate of nonsynonymous mutations minus rate of synonymous mutations,

Gene	N	S	H	Hd ± SD	π ± SD	dN–dS ± SE	Tajima’s D	Fu & Li's D*	Fu & Li's F*
PkCSP	123	83	73	0.978 ± 0.005	0.01929 ± 0.00058	-0.023 ± 0.012	-1.453	-4.78 ¥	-4.03 ¥
PkCSP (Th2R/Th3R)	123	24	27	0.8751± 0.005	0.0293 ± 0.00124	0.004± 0.020	-0.39778	-2.36*	-1.89

^¥ = p < 0.05