Table 1. Primer sequences of ROS scavenging genes used in qRT-PCR.
Proposed gene names, GenBank accession numbers, best BLAST hit names, E-values, sequence identity scores are also listed.
| Gene | Accession number | Best BLAST hit | E-value | Identity | Primer sequence | |
|---|---|---|---|---|---|---|
| CuZn-SOD | DV867103 | JQ269675.1 (Triticum aestivum) | 3e-161 | 87% | Forward | CACTGGACCTCACTTCAAC |
| Reverse | GTAGCAACACCATCCACTC | |||||
| POD2 | DV867327 | XM_010230345.1 (Brachypodium distachyon) | 6e-153 | 89% | Forward | CTTCGACAACGCCTACTAC |
| Reverse | TTTGCCCATGTTCACCA | |||||
| CAT1 | DY543619 | AJ634002.1 (Schedonorus arundinaceus) | 0 | 93% | Forward | TTGCCAATAAGAGGGAGAATG |
| Reverse | CGAAGCCGAGCATGTAAG | |||||
| APX2 | GR281667 | KP852178.1 (Beckmannia syzigachne) | 0 | 94% | Forward | AGGACATTGTTGCCCTTTC |
| Reverse | GCTCCGTGAAGTAAGAGTTG | |||||
| GR | AB277097 | AB277097 (Hordeum vulgare) | 0 | 100% | Forward | GATGGAGGCTACTTGCTTTG |
| Reverse | GCTAAGACCCACGACAGATA | |||||
| MR | DV865077 | KC884831.1 (Triticum aestivum) | 5e-160 | 90% | Forward | CCATGAAGCTCTACAACGAG |
| Reverse | GTAGAAGTAGGGCAGGTAGT | |||||
| DR | DV853556 | HM125046.1 (Puccinellia tenuiflora) | 0 | 90% | Forward | GAAAGGTGCCTGTGTTTAATG |
| Reverse | GTGATGGAGTTGGGTACTTC | |||||
| ACT2 | DY543529 | XM_003578821.2 (Brachypodium distachyon) | 0 | 93% | Forward | CCTTTTCCAGCCATCTTTCA |
| Reverse | GAGGTCCTTCCTGATATCCA | |||||