Skip to main content
. 2015 Sep 18;10(9):e0138181. doi: 10.1371/journal.pone.0138181

Fig 1. A light inducible transcription system.

Fig 1

(A) Schematic representation of the light inducible LexA transcription system. Upon blue light (474nm, ~2.5mWcm-2) illumination, CRY2 undergoes a conformational change that allows it to bind to CIBN to form a functional LexA transcription activator. (B) Light induced GFP expression in S2 cells. S2 cells were transfected with the two constructs that should generate a functional transcription factor upon blue light illumination (pActPL-CIBN-LexA and pActPL-p65-CRY2) and a LexAOP-GFP reporter construct. Cells were exposed to blue light for 1 hour or kept in the dark. (Scale bar = 100μm) (C) Comparison of the efficacy of the VP16 and p65 transcription activation domains. The average number of GFP positive cells per field of view (n = 10, mean±s.e.m. t-test p<0.0001).