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. 2015 Sep 18;10(9):e0138620. doi: 10.1371/journal.pone.0138620

Fig 2. Characterization of iPSCs derived from NSCs of P0-Cre;Z/EG mice (P/G-iPSCs).

Fig 2

AP staining (A), X-gal staining (B), and immunostaining (C; SSEA-1, D; Oct3/4, and E; Sox2) of P/G-iPSCs (clone 61) grown on MEF feeder cells. Scale bars indicate 200 μm (A, B) and 100 μm (C–E). F, RT-PCR analysis of ESC marker gene expression in WT-iPSCs, P/G-iPSCs (clones 23 and 61), and MEFs. Nat1 was used as an internal control. PCR products were amplified from cDNA samples with (+) or without (−) reverse transcriptase.