Fig 3. Recombinant 16E6E7 and 16E6E7m influenza viruses elicit HPV-specific CTL responses in mice.
Four groups of mice (4 per group) were primed with PBS (mock), H1N1 16E6E7 virus, H1N1 16E6E7m virus, parental H1N1 delNS1 virus, or 16L1/L2-E7 VLPs, and boosted 10 days later with PBS, corresponding H3N2 serotypes, or VLPs. Mice were sacrificed A 10 days or B 30 days after boosting, splenocytes were isolated and stimulated in triplicates for 24 h with antigen peptides, SEA or medium alone. For mock and influenza A virus-vaccinated animals, NP311-325 peptide, for mice immunized with 16L1/L2-E7 VLP, 16L1165-173 peptide were used as positive controls. Shown are numbers of IFN-γ spots, counted under a light microscope, and plotted as mean ± SD of triplicate wells. One representative experiment of two is shown. Statistically significant differences for 16E6E7 or 16E6E7m compared to mock are indicated as asterisks (*** p<0.001, ** p<0.01, * p<0.05, ns not significant).