Fig 4. Mice s.c. vaccinated with recombinant 16E6E7 or 16E6E7m influenza viruses are partially protected from TC-1 induced tumors.
A Schematic illustration of the experimental set up. B Groups (n = 8) of C57BL/6 female mice were vaccinated s.c. with PBS (mock), H1N1 16E6E7, H1N1 16E6E7m, parental virus (delNS1), or HPV16 L1/L2-E7 VLP, boosted 20 days later either with PBS, the corresponding H3N2 influenza serotype, or VLPs and challenged s.c. with 5x104 TC-1 cells 10 days later. Animals were monitored once per week. Mean tumor volumes ± SEM of individual animals of one representative experiment of two are shown. Statistical significances of differences recorded for immunized groups and mock treated animals, or immunized groups and parental virus treated animals, were calculated by 1-way ANOVA and subsequent Dunnett’s multiple comparison test. Days after TC-1 tumor inoculation are indicated.