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. 2015 Sep 18;10(9):e0137955. doi: 10.1371/journal.pone.0137955

Fig 5. Effect of RORα deficiency on PEPCK expression.

Fig 5

Suppression of endogenous RORα by siRNA significantly decreased PEPCK mRNA in HepG2 cells. HepG2 cells were transfected with 50 nM siRNA, and analyzed by qRT-PCR to measure expression of RORα and PEPCK. siRNAs that target sequences around 258 bp (siRORa-258, dotted bars) and 1388 bp (siRORa-1388, hatched bars) downstream of the RORα start codon decreased RORα and PEPCK mRNA levels, while a scrambled siRNA (siRORa-mt, closed bars) did not. siRNA against green fluorescent protein was used as negative control (siGFP, open bars). Data are mean ± S.E. of three experiments, and are normalized to 18S rRNA. *, p < 0.05.