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. 2015 Sep 18;10(9):e0138956. doi: 10.1371/journal.pone.0138956

Fig 8. Clonal characterizations.

Fig 8

(A) Thermal stabilities of wild-type (WT) fibronectin [80] and Fn3HP are shown. Median (black line), second and third quartiles (gray box), upper and lower inner fences (vertical lines), and outliers (diamonds) are shown for a sampling of clones from binarily diversified traditional libraries [37,66,80] (n = 15, Trad. Bind.) and three populations of the current study: first generation library binders, second generation naïve library, and second generation library binders. (B) Affinity titrations. Yeast displaying Fn3HP variants were incubated with the indicated concentration of biotinylated target molecule. Binding was detected by streptavidin-fluorophore and flow cytometry. Data points are from a single representative experiment. Affinities were calculated as 150 ±60 pM (rabbit IgG, clone 0.6.2, black triangles), 4 ±3 nM (lysozyme, clone 0.6.3, gray squares), and 11 nM (MET, clone 3.4.3, white circles).