Table 2. Amino acid diversity encoded in second generation library.
CDR’ refers to a degenerate codon with the following nucleotide frequencies: 20% A, 15% C, 25% G, and 40% T at site 1, 50% A, 25% C, 15% G, and 10% T at site 2, and 0% A, 45% C, 10% G, and 45% T at site 3. Loop length diversity was afforded at mid-loop positions by inserting CDR’ diversity sites between sites P25-V29 and G79-S85 of the BC and FG loop, respectively, as denoted by subscripts within table. Length diversity at the DE loop occurred between S53-S55, consisting of either wild-type length or the exclusion of K54.
| BC Loop | DE Loop | FG Loop | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Site | D23 | A24 | P25 | AVT 26–28 | V29 | R30 | Y31 | G52 | S53 | K54 | S55 | T56 | T76 | G77 | R78 | G79 | DSPAS 80–84 | S85 | K86 |
| Generation 2 | D | A/ASYDNT | P/PSYH | CDR‘ 2–4 | AST | CDR' | GY | G | SYNT | (NSYT) 0–1 | SYNT | TSYN | TSGA | GSYADCNT | CDR' | GSDN | CDR’ 1–5 | S | N |