Table 1. Kinetic parameters for PpOpnDH.
Substrates | Electron acceptors | Specific activity a (units/mg protein) | K m | k cat | k cat/K m |
---|---|---|---|---|---|
(mM) | (min-1) | (min-1·mM-1) | |||
Nopaline | Cl2Ind | 27.9±1.3 | 0.476±0.047 | 18900±1700 | 39800±370 |
Nopaline | PMS/INT | 6.45±0.52 | 0.127±0.007 | 5490±320 | 43100±700 |
Nopaline | PMS/NBT | 2.11±0.03 | 0.0772±0.0041 | 1390±40 | 18100±440 |
Nopaline | Ferricyanide | 7.29±0.42 | 0.379±0.051 | 1040±47 | 2750±250 |
Nopaline | Cytochrome c | 18.5±0.24 | 0.379±0.063 | 17920±2800 | 47300±380 |
Octopine | Cl2Ind | 0.00780±0.00074 | 39.0±15.6 | 138±49 | 3.60±0.27 |
a Under standard assay conditions described in the Materials and Methods section.