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. 2015 Sep 18;10(9):e0138434. doi: 10.1371/journal.pone.0138434

Table 1. Kinetic parameters for PpOpnDH.

Substrates Electron acceptors Specific activity a (units/mg protein) K m k cat k cat/K m
(mM) (min-1) (min-1·mM-1)
Nopaline Cl2Ind 27.9±1.3 0.476±0.047 18900±1700 39800±370
Nopaline PMS/INT 6.45±0.52 0.127±0.007 5490±320 43100±700
Nopaline PMS/NBT 2.11±0.03 0.0772±0.0041 1390±40 18100±440
Nopaline Ferricyanide 7.29±0.42 0.379±0.051 1040±47 2750±250
Nopaline Cytochrome c 18.5±0.24 0.379±0.063 17920±2800 47300±380
Octopine Cl2Ind 0.00780±0.00074 39.0±15.6 138±49 3.60±0.27

a Under standard assay conditions described in the Materials and Methods section.