Table 2. Kinetic parameters for BjOpnDH2.
| Substrates | Electron acceptors | Specific activity a (units/mg protein) | K m | k cat | k cat/K m |
|---|---|---|---|---|---|
| (mM) | (min-1) | (min-1·mM-1) | |||
| Octopine | Cl2Ind | 0.504±0.047 | 19.5±4.4 | 4370±945 | 224±2 |
| Octopine | PMS/INT | 0.555±0.035 | 5.30±0.35 | 1490±91 | 281±2 |
| Octopine | PMS/NBT | 0.243±0.015 | 3.99±0.75 | 533±96 | 134±1 |
| Octopine | Ferricyanide | 0.889±0.052 | 11.8±1.0 | 4730±406 | 401±1 |
| Octopine | Cytochrome c | 0.919±0.041 | 3.63±0.06 | 1740±14 | 479±4 |
| Nopaline | Cl2Ind | 0.001> | N.D. b | N.D. | N.D. |
a Under standard assay conditions described in the Materials and Methods section.
b Not determined due to trace activity.