Fig. 2.

Para-nucleolar poly-GA inclusions colocalize with transcriptionally silenced DNA. Immunofluorescence for poly-GA proteins with the indicated antibodies and dyes to label DNA or RNA in frontal cortex of cases with C9orf72 mutation. a Para-nucleolar poly-GA inclusions are enriched for heterochromatin labeled with the DNA-specific dye DAPI (arrow). b Para-nucleolar poly-GA inclusions are also stained with RNA-selective dyes SYTO12 and SYTO RNAselect. Note that both dyes also show chromatin staining similar to DAPI indicating cross-reactivity with DNA. c Nuclear poly-GA inclusions colocalize with histone 3 dimethylated at lysine 9 (H3K9me2), a marker for transcriptionally inactive DNA. Scale bars represent 10 µm