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. 2015 Sep 19;17(1):257. doi: 10.1186/s13075-015-0775-2

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© Choulaki et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Induction of nucleotide binding domain and leucine-rich repeat pyrin 3 domain (NLRP3)-inflammasome related proteins in peripheral whole blood cells, upon sequential stimulation with lipolysaccharide (LPS) (250 pg/mL, 2 h) and ATP (5 mM, 20 min), in healthy individuals and patients with rheumatoid arthritis (RA). Western blots were performed in freshly isolated whole blood cells without (−) or with (+) LPS + ATP treatment from healthy controls (a) (n = 7) and patients with active RA (b) (n = 7). Protein densitometry was assessed using Image J program and the intensity of the protein of interest was normalized to that of β-actin (c-h). HC healthy controls