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. 2015 Sep 18;22(1):79. doi: 10.1186/s12929-015-0179-2

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© Liu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — BDA injection sites. a & b Camera lucida drawings from saggital brainstem sections that contain a BDA deposit in the parabrachial nucleus (a & b). c-g Microphotographs of a BDA injection site are shown in low-power (c: Nova Red; d: Nissl-stained) and magnified views (e & h: Nova Red; f & g: Nissl-stained) The nomenclature for the divisions of subdivisions of the parabrachial nucleus was adapted from Sarhan et al. [21]. Note that the injection site is centered in the central lateral (cl) subnuclus of the parabrachial nucleus. cl, central lateral subnucleus; dl, dorsal lateral subnucleus; el, external lateral subnucleus; em, external medial subnucleus; lcr, lateral crescent subnucleus; ll, lateral lemniscus; LPB, lateral parabrachial nucleus; m, medial parabrachial nucleus; mel, external lateral subnucleus of the mesencephalic parabrachial nucleus; Mo5, trigeminal motor nucleus; scp, superior cerebellar peduncle; scpd, superior cerebellar peduncle, descending limb; sl, superior lateral subnucleus of the mesencephalic parabrachial nucleus; vl, ventral lateral subnucleus; vsc, ventral spinocerebellar tract; 7n, facial nerve; 7, facial nucleus. Scale bar = 500 μm (a-d), 150 μm (e-h)