Fig. 2.

Normalized expression analysis for gene candidates potentially involved in BIA biosynthesis in Papaveraceae (tribe: Papaveroideae) species. Each candidate is labeled with respective species abbreviations (e.g. AME, Argemone mexicana) and the type of enzyme potentially encoded by the gene (e.g. BBE, berberine bridge enzyme). Candidates present exclusively in Roche-based transcriptomes could not be assigned an FPKM value, and are marked with asterisk. Refer to Table 1 for species abbreviations. Enzyme/protein family abbreviations: BBE, berberine bridge enzyme; COR, codeinone reductase; CXE, carboxylesterase; CYP, cytochrome P450 monooxygenase; DIOX, dioxygenase; FAD, FAD-dependent oxidase; NCS, norcoclaurine synthase; NMT, N-methyltransferase; NOS, noscapine synthase; OAT, O-acetyltransferase; OMT, O-methyltransferase; SALR, salutaridine reductase; SANR, sanguinarine reductase