Table 3.

Relative conversion of five alkaloids tested as potential substrates for six NMT candidates from Glaucium flavum

Substrate	Relative activity (% maximum)
	GFLNMT1	GFLNMT2	GFLNMT3	GFLNMT4	GFLNMT5	GFLNMT6
(S)-Coclaurine	100 ± 1a	nd	nd	100 ± 10d, e	<1	100 ± 14g
(S)-Reticuline	nd	nd	100 ± 5c	8 ± 1	100 ± 5f	nd
(S)-Canadine	nd	66 ± 1	<1	nd	nd	nd
(S)-Stylopine	nd	100 ± 2b	81 ± 18	nd	nd	nd
(+/−)-Pavine	nd	<1	nd	nd	nd	nd

Values represent the mean ± standard deviation of three independent assays. For each enzyme, activity was calculated relative to the assay showing the highest conversion of substrate (i.e. the average of this assay was set to 100 %). The accompanying footnote defines 100 % conversion in pmole min⁻¹ mg⁻¹ protein for each enzyme

^a5.9 pmole min⁻¹ mg⁻¹ protein

^b61 pmole min⁻¹ mg⁻¹ protein

^c0.4 pmole min⁻¹ mg⁻¹ protein

^dProducts were N-methylcoclaurine and N,N-dimethylcoclaurine

^e2.3 pmole min⁻¹ mg⁻¹ protein

^f 0.1 pmole min⁻¹ mg⁻¹ protein

^g3.3 pmole min⁻¹ mg⁻¹