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. 2015 Sep 18;16(1):711. doi: 10.1186/s12864-015-1909-2

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© Ding and Löfstedt. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Functional assay of putative acetyltransferase genes. Y-axis represents the total amount of acetate esters (sum of Z5-10:OAc, Z7-12:OAc, and Z9-14:OAc) produced by the yeast cells transformed with candidate genes (±95 % confidence interval, n = 3). Negative control is yeast cell (∆ATF1) transformed with empty vector and the yeast strain overexpressing ATF1 gene serves as positive control. None of the 34 candidate genes produces significantly higher amount of acetate esters compared to the negative control (overlapping 95 % confidence intervals), whereas the ATF1 produces a 45-fold increase in acetate production