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. 2015 Sep 18;16(1):711. doi: 10.1186/s12864-015-1909-2

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© Ding and Löfstedt. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Functional characterization of the highest expressed desaturase gene, Ase_5534, in yeast expression system. GC-MS analyses of methanolyzed lipid extracts from yeast transformed with empty plasmid pYEX-CHT (a) and pYEX-CHT-Ase_5534 (b). Double bond position of the unsaturated palmitate was confirmed by DMDS derivatization (c, d)