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. 2015 Sep 19;13:308. doi: 10.1186/s12967-015-0642-6

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© Ti et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Unsupervised hierarchical cluster analysis of miRNA expression in LPS pre-Exo uncovers let-7b as a regulator of TLR4 in macrophage polarization. a miRNA expression profiling on total RNA isolated from un-Exo (n = 3) and LPS pre-Exo (n = 3). A heatmap was generated after supervised hierarchical cluster analysis. Differential miRNA expression is shown by red (upregulation) versus green (downregulation) intensity (LPS pre-Exo versus un-Exo, twofold change, p < 0.05). b Validation of unique miRNAs in LPS pre-Exo using real-time RT-PCR. c Real-time RT-PCR analysis of let-7b expression in treated THP-1 cells at 48 h. Data are presented as the mean ± SEM of three separate experiments. *Compared with Con, p < 0.05; ^#compared with HG, p < 0.05. d Immunofluorescence staining displayed TLR4 activity in treated THP-1 cells at 48 h