Fig. 6.

Let-7b is involved in LPS pre-Exo modified macrophage polarization by the TLR4/NF-κB/STAT3/AKT signaling pathway. THP-1 cells were treated with LPS pre-Exo and transfected with the let-7b mimics, let-7b inhibitor or the AKT inhibitor, LY294002. a Levels of TLR4, p-P65, NF-κB, p-STAT3, STAT3, p-AKT, AKT protein were detected by western blotting with the respective antibodies. b The distribution of macrophage subtype M1 (iNOS green) and M2 (Arg1 red) were measured by immunofluorescence. Scale bar 50 μm