Figure 1.

Behavioral change and spawning. Male and female zebrafish were exposed to 25 nM of sex steroids (E2 and 11-KT) and 20, 50 and 100 nM of prostaglandins (PGE₂ and BW245C) separately for 8 days then kept together to analyze any change in behavior. Kruskal–Wallis test followed by Dunn’s post-test was performed to determine any change in normal behavior between the control and treated groups (*p < 0.05). Mann–Whitney U-test was used to determine statistically significant differences between control and KT exposed females paired to untreated females (*p < 0.05). The steroid hormones experiments were performed with 24 fish while 12 fish were used for prostaglandin exposures.