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. 2015 Aug 13;11:23. doi: 10.1186/s12993-015-0068-6

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© Pradhan and Olsson. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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qRT-PCR array. Male and female zebrafish (n = 4 for each sex) were exposed separately to 25 nM E2 for 24 h and the brain tissue was isolated and dissected into three regions. RNA was isolated from individual samples followed by qRT-PCR analysis of 32 genes. a Tableau software was used to generate the heat map. The scale bar represents magnitude of expression with male sample from forebrain set to 1 and highest expression fixed to 4. b Venn-diagram showing the sexually dimorphic genes with higher expression of 8 genes in females while only 5 genes were male biased. 19 genes did not show sexually dimorphic expression patterns. c Venn-diagram showing E2 mediated dimorphic gene expression with six genes being regulated in males only, six genes being regulated in both sexes and one gene being regulated in females only.