FIG. 4.

Changes in TSP-1 expression in conjunctival fibroblasts mediated by IL-1β stimulation. (A) Detection of TSP-1 in culture supernatants as determined by ELISA. (B) TSP-1 mRNA levels normalized to GAPDH levels and referenced to control conditions, determined by quantitative polymerase chain reaction (qPCR). Ten percent serum-supplemented conditions are indicated by 10% fetal bovine serum (FBS) and serum-free conditions by FBS-free. Values are expressed as mean±SEM from 3 independent experiments. Statistically significant differences, when compared with untreated control cells, are indicated with asterisks (t-test; *p≤0.05, **p≤0.01).