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. 2015 Sep 1;31(7):419–428. doi: 10.1089/jop.2015.0029

FIG. 5.

FIG. 5.

Changes in active TGF-β2 levels and CD36 mRNA levels in conjunctival fibroblasts stimulated with IL-1β. (A) Levels of active TGF-β2 secreted by conjunctival fibroblasts in serum-free conditions determined by ELISA. Concentration values were normalized to cell numbers determined by the AlamarBlue® assay. (B) Levels of CD36 mRNA normalized to GAPDH levels and referenced to control conditions, determined by qPCR. Ten percent serum-supplemented conditions are indicated by 10% FBS and serum-free conditions by FBS-free. Values are expressed as mean±SEM from at least 3 independent experiments. Statistically significant differences, when compared with untreated control cells, are indicated with asterisks (t-test; *p≤0.05, **p≤0.01). ND, not detected.