Figure 2.

Glioma-specific and efficient infection of Ad5GliomaFF. Cells were infected with Ad5GliomaFF-CMV-GFP or Ad5CMV-GFP at an MOI of 300 VP/cell for 2 hr on glioma cell lines U87 and U251, patient-derived glioma xenograft cell lines GBM43 and GBM6, nonneoplastic cells NHA (normal human astrocytes) and NSC (neuronal stem cells), and other neoplastic cell lines from various anatomical sites: A549 (human lung adenocarcinoma) and MDA-MB 231-BRM2 (brain metastatic breast cancer). The virus-containing medium was then removed and replaced with fresh medium containing 2% FBS. Fluorescence micrographs were taken 72 hr postinfection (a and c), followed by flow cytometric analysis (b and d). (e) Viral infection was done in the same way as described previously except that cells were incubated with VTW peptides for 1 hr before the infection. (f) Infection procedures were done in the same way as described previously with Ad5GliomaFF-CMV-GFP or Ad5PK7-CMV-GFP, followed by flow cytometric analysis. Scale bar, 100 μm. All images are representative of three independent experiments. Means±SEM are plotted. Color images available online at www.liebertpub.com/hum