Figure 4.

The efficient oncolytic activity of Ad5GliomaFF-SurE1. Cells were infected with Ad5GliomaFF-SurE1 or Ad5WT (a) at serially diluted MOI from 0 to 1000 VP/cell for 2 hr on U87MG, U251MG, GBM43, and GBM6 and the virus-containing medium was removed and replaced with fresh medium containing 2% FBS. Eight days after viral infection, cells were fixed and stained with crystal violet. Micrographs were taken with a Nikon digital camera (d5100), followed by dissolution with methanol, and read with a plate reader at OD₅₇₀. Two of representative images are shown. (b) Cells were infected with Ad5GliomaFF-SurE1 or Ad5WT at an MOI of 100 VP/cell for 2 hr on U87, U251, GBM43, and GBM6 and the virus-containing medium was removed and replaced with fresh medium containing 2% FBS. At the time points of 24, 48, 72, and 96 hr, the MTT assay was performed according to the manufacturer's instruction. Each data point and column is the average of three independent replicates. Means±SEM are plotted. Color images available online at www.liebertpub.com/hum