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. 2015 May 21;26(9):635–646. doi: 10.1089/hum.2015.008

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Copyright 2015, Mary Ann Liebert, Inc.

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Figure 5. — Dynamics of viral replication. Cells were infected with Ad5GliomaFF-SurE1 or Ad5WT at an MOI of 100 VP/cell for 2 hr on U87, U251, GBM43, and GBM6. The virus-containing medium was removed and replaced with fresh medium containing 2% FBS. Both virus-infected cells and media were collected 24, 48, and 72 hr after viral infection and the DNA from each sample was isolated, followed by qPCR with primer sets for genes of the replication-dictating E1 (E1A (a) and E1B (b)), viral DNA replication-related precursor terminal protein (pTP (c)), and a structural protein (fiber (d)). Each column represents the average fold change in the respective gene of three independent replicates from Ad5GlimaFF-SurE1 compared with Ad5WT. Means±SEM are plotted.