Effects of the different chronic protocols on oxidative phosphorylation
A, LV mitochondrial BN-PAGE profile of the experimental groups. B, representative images of histochemical staining, with semi-quantitative analysis of in-gel activity of complex IV. C, representative images of histochemical staining, with semi-quantitative analysis of in-gel activity of complex V. D, activity of complex V assayed by spectrophotometry. Five independent experiments assayed in duplicate were considered for analysis. Error bars indicate the mean ± SD. *P < 0.05 vs. Cont; ‡P < 0.05 vs. Ex.