Figure 3.

Ghrelin and leptin or CCK-8 activate different antagonistic currents

A, representative current recording in response to ghrelin (30 nm, upper trace), leptin (10 nm, middle trace), and ghrelin and leptin combined (lower trace). Note that ghrelin generated an outward current (upper trace) that was slowly decaying. However, when ghrelin was paired with leptin, the outward current was reversed. B, representative current recording in response to CCK-8 (30 nm) and, 10 min later, in response to ghrelin (30 nm) and CCK-8 (30 nm). Note that the second application of CCK-8 generated a current that was similar in amplitude but ‘displaced’ by the ghrelin-generated current. The shift from the baseline generated by the first and second applications of CCK-8 was 78 pA and 42 pA, respectively. C, pooled data of maximum current amplitudes generated by ghrelin (n = 5), leptin (n = 5), CCK-8 (n = 5) and ghrelin-leptin/CCK-8 groupings (n = 5).