Figure 4.

Ghrelin stimulates p-PI3K and p-Erk1/2 in the nodose ganglia

A, western blot showing that ghrelin stimulates nodose ganglia p-PI3K in a dose-dependent manner (n = 6). B, western blot showing that ghrelin stimulates nodose ganglia p-Erk1/2 in a dose-dependent manner (n = 6). C, ghrelin (20 nm)-stimulated p-Erk1/2 was inhibited by wortmannin (100 mm) and PTx (1 U ml^–1) but not by PLC inhibitor U73122 (10 μm), PKC inhibitor calphostin C (1 μm), adenylate cyclase SQ 22536 (100 μm) or PKA inhibitor H7 (30 μm) in the nodose ganglia (n = 5–6, *P < 0.05 compared to basal controls; ^#P < 0.05 compared to stimulation by ghrelin). D, Western blot showing ghrelin-stimulated Erk1 and Erk2 mRNA expression in vagal ganglia 96 h after transfection with random siRNA but not with PI3K siRNA [n = 4, *P < 0.05 compared to basal control; **P < 0.05 compared to nodose ganglia transfected with control (random) siRNA].