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. 2015 Sep 1;593(Pt 17):3973–3989. doi: 10.1113/JP270788

Figure 6.

Figure 6

Kir6.2 subunit mediates the inhibitory actions of ghrelin inhibition in vivo

A, single-unit recordings in response to leptin (225 μg kg–1 i.v.) and leptin after ghrelin administration (25 μg kg–1 i.v.) from the right nodose ganglia, which were electroporated with Kir6.2 channel siRNA (lower trace). B, single-unit recordings in response to CCK-8 (30 μg kg–1 i.v.) and CCK-8 after ghrelin administration (25 μg kg–1 i.v.) in the right nodose ganglia, which were electroporated with Kir6.2 channel siRNA (lower trace). C, Kir6.2 mRNA expression (RT-PCR) in vagal ganglia 96 h after electroporation with control (random) siRNA and Kir6.2 siRNA (n = 4, *P < 0.05 compared to control). D, discharges of vagal sensory neurons in response to i.v. injections of leptin and CCK-8 were inhibited by ghrelin in control siRNA-treated animals. The inhibitory actions of ghrelin were abolished in rats whose right nodose ganglia were electroporated with Kir6.2 siRNA (n = 5–6, *P < 0.05 compared to control). E, single-unit discharge in response to leptin (225 μg kg–1 i.v.) and leptin after the administration of ghrelin (25 μg kg–1 i.v.) (upper) or CCK-8 (30 μg kg–1 i.v.) and CCK-8 after the administration of ghrelin (25 μg kg–1 i.v.) (lower) in the right nodose ganglia, which were electroporated with Erk1/2 siRNA. F, single-unit discharge in response to leptin (225 μg kg–1 i.v.) and leptin after the administration of ghrelin (25 μg kg–1 i.v.) in nodose ganglia treated with PI3K siRNA (upper) or CCK-8 (30 μg kg–1 i.v.) and CCK-8 after the administration of ghrelin (25 μg kg–1 i.v.) in the right nodose ganglia, which were electroporated with Erk1/2 siRNA.