Figure 1.

Protocol and representative traces

Mitochondrial membrane potential using TMRM (A), Rate of H₂O₂ emission using Amplex Red, HRP and SOD (B) and respiration (C). Black filled circles (•) indictate when using SR (with malonate as an inhibitor) and open circles (○) indictate when using PM (with rotenone as an inhibitor). The light grey area represents the phosphorylating part of the protocol and the darker grey area represents the non-phosphorylating part. Traces are from an aged trained subject.