Figure 1.

The experimental outline

The screening was conducted over two separate days (screening A and B). If subjects were eligible to be enrolled in the present study after the first screening A, they were invited to screening B. A random leg was immobilized for 2 weeks, whereas the other leg was used to ambulate freely with the use of crutches. Biopsies were obtained from the immobilized leg before (T1IM) and after immobilization (T2IM) and in the previously immobilized leg after the training period (T3IM). Also, a biopsy was obtained from the non-immobilized control leg after immobilization (T2CON).