Table 1. Table of primary antibodies used.
List of the primary antibodies used in the study with their respective specifications.
| Antigen | Description of immunogen | Source, host species, cat. #, clone or lot #, RRID | Working concentration used (µg/ml) |
|---|---|---|---|
| calbindin | purified bovine kidney CB 28 kD | Sigma Chemical Co., cat. # C9848, RRID: AB_476894 | 8.3 |
| cholera toxin β subunit | Ctβ itself | List Biological Laboratories, cat. #703, RRID: AB_10013220 | 15.3 |
| Fluorogold | FG itself | Millipore, cat. #AB153, RRID: AB_90738 | 30.5 |
| Fos | synthetic peptide corresponding to amino acids 4-17 (SGFNADYEASSSRC) of human c-Fos | Calbiochem, cat. # PC38, RRID: AB_2313765 | 11.5 |
| nitric oxide synthase | amino acids 251-270 of nitric oxide synthase (GDNDRVFNDLWGKDNVPVILC) conjugated to keyhole limpet cyanin | Sigma Chemical Company, cat. #N7155, RRID: AB_260795 | 21.5 |
| μ-opioid receptor | synthetic peptide corresonding to amino acids LENLEAETAPLP at the COOH terminus of the μ-opioid receptor | Gramsch Laboratories, cat. # OR-600, RRID: AB_2314811 | 16.0 |
| parvalbumin | purified carp muscle parvalbumin | Sigma Chemical Co., clone PA-235, cat. # P3088, RRID: AB 477329 | 26.3 |
| tyrosine hydroxylase | tyrosine hydroxylase from PC12 cells | Millipore, cat. # MAB318, RRID: AB_2315522 | 28.8 |