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. Author manuscript; available in PMC: 2016 Mar 17.
Published in final edited form as: Nature. 2015 Aug 31;525(7569):345–350. doi: 10.1038/nature14887

Figure 5. CMGs dissociate after dissolution and ligation.

Figure 5

A. LacR block-IPTG release was followed by MCM7, CDC45, RPA and, Polε ChIP at the indicated times after IPTG addition. Dissolution, ligation, and decatenation were measured in parallel. means±s.d. are plotted (n=3).

B. p[empty] was replicated in extracts treated with Vehicle, Ubiqutin-Vinyl Sulfone (Ub-VS), or Ub-VS and free ubiquitin (UbVS + Ub). Dissociation of MCM7 was measured by ChIP (see methods). mean±s.d. is plotted (n=3).

C. Same as (B), but CDC45 dissociation was measured.

D-E. In parallel to MCM7 and CDC45 dissociation (B, C), dissolution (D) and ligation (E) were measured. mean±s.d. is plotted (n=3). See Extended Data Fig. 8F–I for decatenation measurements and representative gels.

(E) New model of vertebrate replication termination.